Fig 1: Blocked S phase and restrained proliferation in KDM5B knockdown GCs. (A) Flow cytometry results show that the percentage of cells in the G1 phase significantly decreases in the KDM5B knockdown goat GCs and the percentage of cells in the S phase increases compared to the NC. Red represents cells in G1 and G2 phases. (B) mRNA expressions of Ccnd1, Ccnd3 and CDK4 increase in the KDM5B knockdown goat GCs compared to the NC, as determined by qPCR. (C) Western blot shows that CDK6, Ccnd1 and Ccnd3 protein levels increase in the KDM5B knockdown goat GCs compared to the NC. (D) Goat GCs treated with siKDM5B show decreased Edu incorporation relative to the NC. Hoechst indicates nuclear staining. The scale bars correspond to 100 μm. (E) mRNA expression of PCNA significantly decreases in the KDM5B knockdown goat GCs relative to the NC. (F) Western blot shows that PCNA protein level significantly decreases in the KDM5B knockdown goat GCs relative to the NC. ** means p < 0.01, * means 0.01 < p < 0.05.